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  À㳪¹« ħ¿± ³» ¿±·Ï¼Ò ÇÔ·® ºñ±³ Chlorophyll Contents of Korean Pine Needles
  ±Û¾´ÀÌ : °ü¸®ÀÚ     ³¯Â¥ : 08-03-11 12:45     Á¶È¸ : 9727    
¾Æ¼¼Åæ°ú DMSO (Dimethylsulfoxide)·Î ÃßÃâÇÏ¿© ÃøÁ¤ÇÑ À㳪¹« ħ¿± ³» ¿±·Ï¼Ò ÇÔ·® ºñ±³ Extraction and Determination of Chlorophyll Contents of Korean Pine Needles Using Acetone and DMSO (Dimethylsulfoxide) <Çѱ¹ÀÓÇÐȸÁö 94(4):264-268 Jour. Kor. For. Soc. 94(4):264-268 2005> Corresponding author yson@korea.ac.kr

Âü³ª¹«·ù ÀӺп¡ ÀÚ¿¬ ¹ß»ýÇÑ À㳪¹«¿Í Àΰø Á¶¸²µÈ À㳪¹« ħ¿±À» 80% acetone°ú DMSO(Dimethylsulfoxide)·Î ÃßÃâÇÏ¿© ÃøÁ¤ÇÑ ¿±·Ï¼Ò ÇÔ·®À» ºñ±³ÇÏ¿´´Ù. 80% acetone¿¡ ºñÇØ DMSO·Î ÃßÃâÇÑ ÃÑ ¿±·Ï¼Ò ÇÔ·®Àº À㳪¹« ħ¿±ÀÇ ¸ðµç ¿¬·É¿¡¼­ ¾à 4-6¹è ³ôÀº °ªÀ» ³ªÅ¸³Â´Ù. ¶ÇÇÑ DMSO·Î ÃßÃâÇÑ °æ¿ì »óÃþ ÀÓ°üÀ» Á¡À¯ÇÑ Àΰø Á¶¸²µÈ À㳪¹«¿Í ÇÏÃþ¿¡ »ýÀ°ÇÏ´Â À㳪¹« °£¿¡´Â ÃÑ ¿±·Ï¼Ò ÇÔ·®¿¡ Â÷ÀÌ°¡ ¾ø¾úÀ¸¸ç, 80% acetoneÀ¸·Î ÃßÃâÇÑ °æ¿ì¿¡µµ ¿ª½Ã µÎ Á¾·ùÀÇ À㳪¹« »çÀÌ¿¡ ¿¬·Éº° ÃÑ ¿±·Ï¼Ò ÇÔ·®ÀÇ Â÷ÀÌ´Â ¾ø¾ú´Ù. DMSO·Î ÃßÃâÇÒ °æ¿ì ÀÚ¿¬ ¹ß»ý À㳪¹«¿Í ÀΰøÁ¶¸²µÈ À㳪¹« ħ¿± ¸ðµÎ 6½Ã°£ÀÇ ÃßÃâÀÌ ÀûÀýÇÑ °ÍÀ¸·Î ÆǴܵȴÙ.
Chlorophyll contents in Korean pine (Pinus koraiensis) needles of an advance growth stand and a plantation were compared using 80% acetone and DMSO (dimethylsulfoxide). Total chlorophyll contents in DMSO were 4-6 times higher than those in 80% acetone. There were no significant differences in chlorophyll contents between two stand types in both 80% acetone and DMSO. It appeared that 6 hours in DMSO would be appropriate to successfully extract chlorophyll from Korean pine needles of advance growth and planted trees.
Key words: 80% acetone, chlorophyll contents, DMSO, Pinus koraiensis

   

 
   

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