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| ▒ Home | > | | 연구정보 (Information) | > | 국내연구정보 (Domestic information) |
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| Stain Technique for Diagnosing Blister Rust |
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| 글쓴이 : 관리자
날짜 : 08-11-14 10:50
조회 : 9679
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Studies on the Control of Korean White Pine (Pinus koraiensis) Blister Rusts (III) -A Stain Technique for Diagnosing Blister Rust of Korean White Pine- <Jour. Kor. For. Soc. 37:61-63 1978> Chong-Jin Kim
Both bark and wood sections obtained, by cutting with a razor blade, from living tissue of
Cronartium ribicola cankers of Korean white pine (Pinus koraiensis) were transferred to a
mixture of 2 parts of chloroform and 1 part of methanol, and the sections were stained
using a modified lactophenol cotton blue. The formula for this staining is as follows: lactic
acid 20mg, phenol crystal 20mg, cotton blue 0.05mg, and 60% EtOH 100ml. The rust
hyphae and haustoria were stained blue, and the wide hyphae with straight or curved
haustoria could be distinguished from the pine tissue.
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