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| ▒ Home | > | | 연구정보 (Information) | > | 국내연구정보 (Domestic information) |
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| 캘러스 세포의 염색체변이 Chromosomal Variation in the Callus |
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| 글쓴이 : 관리자
날짜 : 08-06-26 10:36
조회 : 10050
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In vitro studies on Pinus koraiensis (II) -Chromosomal Variation in the Callus- 잣나무의 기내배양에 관한 연구(II) -Callus 세포의 염색체변이- <한국임학회지 74:56-60 Jour. Kor. For. Soc. 74:56-60 1986> Corresponding author Young Goo Park ygpark@knu.ac.kr
Excised mature embryos of Pinus koraiensis were plated on a series of Gresshoff-Doy media supplemented with growth regulators as follows: 0.1 mg/l NAA (M-1); 0.1 mg/l NAA and 2,4-D (M-2); 0.1 mg/l 2,4-D and BAP (M-3); 0.1 mg,/l 2,4-D and kinetin (M-4). Cytological study of the callus showed that the per centage of occurrence of diploid cells was observed 52% in M-3 and 36% in M-4, while that was revealed 29% in M-1 an 17% in M-2. The frequency of diploid cells was increased on the M-3 and M-4 media. The stable chromosome state is a crucial factor for organogenesis. Therefore, it can be inferred that the callus tissues cultured on media supplemented with both auxin and cytokinin have the greatest possibility of organogenesis.
Key words: chromosomal variation, organogenesis, auxin, cytokinin, Pinus koraiensis
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