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| 피목가지마름병균의 배양특성 및 병원성 검정 Cultural Characteristics and Pathogenicity Test of Die-back Fungus, Cenangium ferruginosum |
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| 글쓴이 : 관리자
날짜 : 08-05-05 10:53
조회 : 9743
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잣나무에서 분리한 피목가지마름병균, Cenangium ferruginosum의 배양특성 및 병원성 검정 Cultural Characteristics and Pathogenicity Test of Die-back Fungus, Cenangium ferruginosum Isolated from Pinus koraiensis <한국임학회지 87(4):557-561 Jour. Kor. For. Soc. 87(4):557-561 19958> Senior author sangyong@kangwon.ac.kr
피목가지마름병에 감염된 잣나무로부터 병원균을 분리 동정한 결과, Cenangium ferruginosum으로 동정되었다. C. ferruginosum의 균사생장은 공시한 5종의 인공배지 중에서 PDA(Potato Dextrose Agar)에서 가장 왕성하였으au, 활엽수 추출물 첨가배지에서보다 침엽수 추출물 첨가배지에서 상대적으로 좋았다. PDA에서의 균사생장 최적온도 및 pH는 각각 10℃ 및 pH 5였다. 한편, 5년생 잣나무에 인공배양한 균사를 접종하여 병원성을 검정한 결과 80%가 고사하였으며, 자연상태에서 감염된 이병목과 동일한 병징을 나타내었다.
A die-back fungus isolated from Pinus koraiensis was identified as Cenangium ferruginosum. Mycelial growth of the fungus was the best on PDA among five media tested. Mycelial growths on agar media supplemented with extracts from coniferous trees were relatively better than those on media with extracts from non-coniferous trees. Optimum temperature and pH for the mycelial growth of C. ferruginosum. on PDA were 110℃ and pH 5, respectively. When five-year-old Pinus koraiensis seedlings were artificially inoculated with the fungus for the pathogenicity test of this fungus, 50% of seedlings were infected, and the same symptoms and signs were developed as those of naturally-infected trees.
Key words: die-back, pathogenicity, Pinus koraiensis, Cenangium ferruginosum, tree extracts
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